Study on the mechanism of production of γ-PGA and nattokinase in Bacillus subtilis natto based on RNA-seq analysis

Abstract Poly-γ-glutamic acid (γ-PGA) and nattokinase (NK) are the main substances produced by Bacillus subtilis natto in solid-state fermentation have wide application prospects. We found that our strains had higher activity of when soybeans were used as substrate to increase yield γ-PGA. Commercial production γ-PGA requires an understanding mechanism co-production. Here, we obtained maximum (358.5 g/kg, w/w) highest NK during analyzed transcriptome co-production NK. By comparing changes expression genes encoding key enzymes metabolic pathways associated with products genetic engineering, can be summarized based on RNA-seq analysis. This study firstly provides new insights into reveals potential molecular targets promote nattokinase.